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AYN ameliorated hypertrophic adipocytes of diabetic mice. (A) HE staining for epWAT; (B) HE staining for SAT; (C) Adipocyte areas of epWAT; (D) Adipocyte areas of SAT. ( n = 6, +++ p < 0.001, compared with Normal control group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Vehicle control group).

Journal: Food Science & Nutrition

Article Title: Anti‐Diabetic Effects of Ayanin, a Flavonoid Compound, in STZ / HFD ‐Induced Diabetic Mice by Upregulating GLUT4 and Suppressing Macrophage‐Driven Inflammation in Adipose Tissues

doi: 10.1002/fsn3.71429

Figure Lengend Snippet: AYN ameliorated hypertrophic adipocytes of diabetic mice. (A) HE staining for epWAT; (B) HE staining for SAT; (C) Adipocyte areas of epWAT; (D) Adipocyte areas of SAT. ( n = 6, +++ p < 0.001, compared with Normal control group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Vehicle control group).

Article Snippet: The 3T3‐L1 fibroblast differentiation into adipocytes was stimulated using different media containing insulin (10 mg/mL, HY‐ P73243 , MedChemExpress, China), dexamethasone (10 μM, HY‐14648, MedChemExpress, China), IBMX (0.5 M, HY‐12318, MedChemExpress, China), indomethacin (125 nM, HY‐14397, MedChemExpress, China), and rosiglitazone (1 μM, HY‐17386, MedChemExpress, China) for 5 days, followed by insulin (10 mg/mL) for 2 days.

Techniques: Staining, Control

AYN increased glucose uptake of 3T3‐L1 adipocytes by activating AMPKα/GLUT4 pathway. (A) AYN increased GLUT4 and p‐AMPKα expression in 3T3‐L1 adipocytes; (B) Relative band intensity for GLUT4/β‐Actin, p‐AMPKα/AMPKα for protein in 3T3‐L1 adipocytes; (C) AYN increased the glucose uptake of 3T3‐L1 adipocytes; (D) AMPKα inhibitor, Compound C, suppressed the GLUT4 expression of 3T3‐L1 adipocytes induced by AYN; (E) Compound C inhibited the glucose uptake of 3T3‐L1 adipocytes induced by AYN. ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Normal control group; +++ p < 0.001, compared with AYN group in E).

Journal: Food Science & Nutrition

Article Title: Anti‐Diabetic Effects of Ayanin, a Flavonoid Compound, in STZ / HFD ‐Induced Diabetic Mice by Upregulating GLUT4 and Suppressing Macrophage‐Driven Inflammation in Adipose Tissues

doi: 10.1002/fsn3.71429

Figure Lengend Snippet: AYN increased glucose uptake of 3T3‐L1 adipocytes by activating AMPKα/GLUT4 pathway. (A) AYN increased GLUT4 and p‐AMPKα expression in 3T3‐L1 adipocytes; (B) Relative band intensity for GLUT4/β‐Actin, p‐AMPKα/AMPKα for protein in 3T3‐L1 adipocytes; (C) AYN increased the glucose uptake of 3T3‐L1 adipocytes; (D) AMPKα inhibitor, Compound C, suppressed the GLUT4 expression of 3T3‐L1 adipocytes induced by AYN; (E) Compound C inhibited the glucose uptake of 3T3‐L1 adipocytes induced by AYN. ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Normal control group; +++ p < 0.001, compared with AYN group in E).

Article Snippet: The 3T3‐L1 fibroblast differentiation into adipocytes was stimulated using different media containing insulin (10 mg/mL, HY‐ P73243 , MedChemExpress, China), dexamethasone (10 μM, HY‐14648, MedChemExpress, China), IBMX (0.5 M, HY‐12318, MedChemExpress, China), indomethacin (125 nM, HY‐14397, MedChemExpress, China), and rosiglitazone (1 μM, HY‐17386, MedChemExpress, China) for 5 days, followed by insulin (10 mg/mL) for 2 days.

Techniques: Expressing, Control